日本结缕草茎节和愈伤组织再生体系的优化

分别以日本结缕草的成熟胚和茎节为外植体,建立并优化了2套组培再生体系.实验结果表明:利用成熟胚诱导愈伤组织,适宜诱导培养基为MS基本培养基添加2 mg/L 2,4-D配合0.2 mg/L 6-BA,诱导率为42.5%;继代培养可改善愈伤组织状态,增加胚性,其中增加培养基渗透压,并适当添加ABA(0.2 mg/L),可明显提高胚性愈伤组织比率;继代2次后的愈伤组织在3种细胞分裂素6-BA,KT,ZT适当配比的分化培养基上,分化率可达到50%~75%.在以茎节为外植体的组培体系中消毒处理和预培养是比较关键的环节;预培养时MS培养基效果优于1/2 MS,在MS基本培养基中添加0.25 mg/L KT,1.0 mg/L ZT和0.1 mg/L NAA为比较适合的芽诱导培养基,诱导率可达37.9%.同时,比较了两套再生体系的优缺点,为日后日本结缕草的遗传转化奠定基础.     

河南两产地日本三角涡虫的染色体和核型多样性分析

利用空气干燥法对采自河南省桐柏县西小河和三里岔村两产地日本三角涡虫(Dugesia japonica)的染色体和核型进行了研究,结果表明:日本三角涡虫西小河种群的体细胞中染色体数目以16条为主,少数为24条和25条,分别占86.67%、6.07%和7.26%,染色体基数为8,为2倍体、3倍体和3倍性非整倍体的混合体,核...     

海带对罗源湾养殖区海水的生物修复研究

研究了罗源湾海带栽培期间3-5月的水质状况,以及室内海带个体对养殖废水的净化作用.结果表明:大面积海带栽培可改善罗源湾的水质状况,降低无机氮磷含量.3-5月海带栽培区的总无机氮(DIN)的降低率分别达到25.48％、19.62％、44.65％,总无机磷(DIP)的降低率分别为20.17％、32.00％、38.72％.在7d实验期间,海带个体对养殖废水的净化效果明显,DIN的去除率可达98％以上.海带在养殖废水中能正常生长,保持一定的日增长率,鲜质量及叶片长有所增加.     

Effects of fragmentation on genetic variation in populations of the terrestrial earthworm Drawida japonica Michaelsen, 1892 (Oligochaeta,Moniligastridae) in Shandong and Liaodong peninsulas,China

Earthworms are excellent indicators of landscape fragmentation due to their widespread distribution and poor dispersal abilities over water. Based on field studies conducted in the Shandong and Liaodong peninsulas and their associated islands during summer 2009, we analysed the genetic variation of Drawida japonica Michaelsen, 1892 (Oligochaeta, Moniligastridae) using DNA sequences of the 16S and 28S ribosomal genes in samples obtained from 13 localities, 12 of which were islands, one artificially connected to the mainland by man (Yangma SC). We identified 55 haplotypes among 79 samples, based on 1094 bp, including 47 unique haplotypes. The phylogenetic tree topologies showed two distinct clades. A multiple-apex Poisson distribution using a mismatch analysis of nucleotides was also detected. Results of a Tajima D neutral test were not significant (p > 0.05). Genetic divergence among populations was much larger than within populations. A positive relationship between genetic diversity and island area was revealed, but no significant correlation between genetic diversity and distance to the nearest larger landmass was found. These results indicate that the intraspecific genetic diversity of D. japonica has been influenced by geographic isolation (i.e. island habitat). Passive transport via anthropogenic activities or nonhuman vertebrates (birds or mammals) may also play a key role in dispersal.     

日本林蛙的器官发育：呼吸,循环,尿殖系统和感觉器官的发育

通过对日本林蛙早期胚胎发育的观察和出膜至变态蝌蚪、幼蛙及成体的系统解剖,对呼吸,循环、泌尿、生殖系统和感觉器官的发生,发展与变化过程进行了描述和讨论.     

内蒙古孩儿参属的研究

本文确认了内蒙古境内分布的6种孩儿参属Psendostelaria Pax植物,比1978年出版的《内蒙古植物志》第二卷增加了4种,其中P.japonica(Korsh.)Pax、P.rupetris(Turcz.)Pax、P.maximowicziana(Franch.et Sav.)Pax为內蒙古分布新记录种,而P.helanshanensis W.Z.Di et Y.Ren为新种收载种。本文否定了新种P.termnialis W.Z.Di et Y.Ren它应为P.ropestris(Turez.)Pax的新异名。     

4种变温动物松果腺复合体的形态结构比较

本文采用光镜和透射电镜，对日本七鳃鳗，黑斑蛙和丽斑麻蜥４种变动物的松果腺复合体，进行了观察和比较，并对其结构与功能的关系作了初步探讨，变温动物的松果腺复合体，由副松果体和松果体构成，在系统发育中，副松果体始终是光感受器，松果体则从光感觉器向着内分泌腺发展，从这４种动物松果腺复合体的变化揭示；最早脊椎动物的松果眼是２个，以后在动物从低等向高等进化中，其中一个保留在变温动物，始终是光感觉器，另一个变成     

利用污泥建植结缕草草坪及生产结缕草草皮卷的试验研究

研究了不同污泥配比建植结缕草草坪、生产结缕草草皮卷的效果，结果表明污泥对结缕草的生长有很好的促进作用，其最佳配比为：50％污泥＋40％土＋10％沙。各处理间对草皮卷韧性的影响效果不显著。     

Transformation of japonica rice with RHL gene and salt tolerance of the transgenic rice plant

Overexpression of the yeast HAL2 gene increases salt tolerance of yeast and plant. Rice HAL2-like (RHL) gene was introduced into a japonica rice cultivar HJ19 with Agrobacterium tumefaciens-mediated transformation. Transgenic plants in R0 generation were selected on the principle of GUS-positive, RHL gene PCR-positive and normal growth. Hygromycin-resistant plants of some transgenic lines in R1 generation increased salt tolerance during the seedling and booting stage, being less damaged in the cytomembrane and stronger in leaf tissue viability under salt stress during booting period. Southern analysis of transgenic lines tolerant to salt in R1 generation showed that the RHL gene expression cassette had been successfully integrated into rice genome. Moreover, gene engineering breeding methodology and really salt-tolerant rice cultivar were discussed.     

中华绒螯蟹卵巢新基因EJO6的全长cDNA克隆和序列分析

利用RACE技术从中华绒螫蟹卵巢获得了新基因EJO6(Eriocheir japonica ovary gene 6,EJO6)的全长cDNA序列(GenBarnk 检索号：AYl85922)。该cDNA序列长度为1250bp,开放阅读框为690bp,编码229个氨基酸。根据氨基酸序列计算的相对分子质量和等电点分别为24270和11．9。同时用生物信息学的方法对该基因的结构和功能进行了初步分析。